Human HLA-Expressing Mouse Strains

Reagent Descriptions
Two reagents are available:

• HLA-A2.1: This transgenic mouse strain was constructed by microinjection of the 7kb EcoRI fragment containing the full-length HLA-A2.1 gene into fertilized eggs from C57BL/6 mice. The strain expresses human class I MHC Ag HLA-A2.1 on cells from the spleen, bone marrow and thymus. A full description can be found at the Jackson Laboratory, where this strain is sold as stock number 003475. Use of this strain by companies or for-profit entities requires a license from the U.Va. Patent Foundation.

• HLA-AAD: This strain was constructed by microinjection of a transgenic construct containing the leader, alpha-1 and alpha-2 domains of the human HLA-A2.1 and the alpha-3 transmembrane and cytoplasmic domains of mouse H-2D into fertilized (C57BL/6 X CBA/Ca)F1 mouse eggs. Founder animals were bred with C57BL/6 mice. Strain expresses the interspecies hybrid class I MHC gene, AAD, under the direction of the human HLA-A2.1 promoter. A full description can be found at the Jackson Laboratory, where this strain is sold as stock number 004191. Use of this strain by companies or for-profit entities requires a license from the U.Va. Patent Foundation.

Strain Names
C57BL/6-Tg(HLA-A2.1)1Enge/J
B6.Cg-Tg(HLA-A/H2-D)2Enge/J

References
Immunol. 1996 Apr 1;156(7):2473-80. (Available online.)