Description:
Production and Reactivity
These mouse monoclonal antibodies were generated against bovine SMARCAL1 and recognize the species indicated below in addition to the bovine homolog. No antibody clone neutralizes DNA-dependent ATP hydrolysis. The relative reactivity to human SMARCAL1 by WB is 5A12 > 4B10 > 10H7 > 5G4 > 6E12 > 2D1.
Clone
|
Isotype
|
ELISA
|
WB
|
IF
|
IP
|
Human
|
Mouse
|
2A3 |
IgG1
|
Y
|
Y
|
|
Y
|
|
|
5G4 |
IgG1
|
Y
|
Y
|
|
Y
|
Y
|
Y |
1H10 |
IgG2a
|
Y
|
Y
|
|
Y
|
|
|
5A12 |
IgG1
|
Y
|
Y
|
|
Y
|
Y
|
Y |
3B2 |
IgG1
|
Y
|
Y
|
|
Y
|
|
|
7B1 |
IgG1
|
Y
|
Y
|
|
Y
|
|
|
10H7 |
IgG1
|
Y
|
Y
|
|
Y
|
Y
|
Y |
10E8 |
IgG1
|
Y
|
Y
|
|
Y
|
Y
|
|
4B10 |
IgG1
|
Y
|
Y
|
|
Y
|
Y
|
Y |
6E12 |
IgG1
|
Y
|
Poor
|
Y
|
Y
|
Y
|
Y |
2D1 |
IgG1
|
|
Poor
|
|
Y
|
Y
|
Y |
6E2 |
Mixed*
|
|
Y
|
|
Y
|
|
|
4H11 |
Mixed*
|
|
Y
|
|
Y
|
|
|
*IgG1, IgG2a and IgA
References
Biochemistry. 1993 Aug 3;32(30):7772-8.
Biochemistry. 1991 Dec 10;30(49):11490-4.