Description:
Description
The isotype-defining domain (C-terminal 16 amino acids) of each class of human beta-tubulin was fused to Maltose Binding Protein (MBP) and expressed in bacteria. The fusion proteins* are expressed in high amounts, can be purified by maltose chromatography and are very soluble in aqueous buffers. The proteins can be used as a standard in quantitative western blotting, dot blotting and ELISA.
References
Methods Cell Biol. 2010;95:33-46. (Available online.)
*The fusion proteins are expressed from a modified pMal-C2 vector. Commercial use of the fusion proteins may require a license from New England Biolabs.
