Description:
Reagent Description
These mutant mice may be useful in studies of T cell activation, T cell receptor stimulation and TGF-beta signaling. A targeting vector was designed to replace exon 1 of the endogenous gene with a lacZ gene (with a nuclear localization signal) and a loxP-flanked PGK-neo cassette. This construct was microinjected into (129X1/SvJ x 129S1/Sv)F1-derived R1 embryonic stem (ES) cells. Correctly targeted ES cells were injected into C57BL/6 blastocysts, which were then implanted into pseudopregnant foster mothers, resulting in heterozygous offspring. These mice were then backcrossed to C57BL/6 for seven generations.
Mice homozygous for the targeted mutation (called “Snoex1” in the primary reference) are viable and fertile with no reported gross morphological defects. Homozygotes exhibit T cell proliferation/activation defects, which can be rescued by treatment with anti-TGF-beta antibodies or exogenous interleukin-2.
A full description can be found at the Jackson Laboratory, where this strain is sold as stock number 006497. Use of this strain by companies or for-profit entities requires a license from the U.Va. Patent Foundation.
Strain Name
B6.129-Skiltm2Spw/J
References
Mol Cell Biol. 2003 Aug;23(15):5446-59. (Available online.)
